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DOI: http://dx.doi.org/10.7551/978-0-262-31709-2-ch118
Page 824
First published 2 September 2013

In vitro Synthesis of Membrane Protein Machinery toward the Construction of Artificial Cell

Hideaki Matsubayashi, Yutetsu Kuruma, Takuya Ueda

Abstract (Excerpt)

Cell membranes are essential part of living cells. They are important as the envelope which encapsulate the biochemical systems within them and distinguish "self" components from "not-self" surrounding environment. Furthermore, cell membranes function as an interface which exchange materials between inside and outside of the cell, sense external environment, and transmit signals to inside systems to response the circumstance. In the field of synthetic biology, liposome (lipid membrane vesicle) has been widely used as a model of cell membrane. Although liposome is certainly a good model as for cell envelope, it is not satisfying the biochemical functions of cell membranes. Since the most of cell membrane functions are led by membrane embeded proteins, we should combine membrane proteins with liposome to construct more feasible artificial cell membranes. In this research, we aim to equip membrane machinery on liposome membrane using in vitro gene expression system.