Microarrays for the Neurosciences
An Essential Guide
A guide to the use of DNA microarray technology in studying the central nervous system and other complex biological systems.
The effort to sequence the human genome has generated a new discipline, “functional genomics,” or the study of the relationship between the genetic code and its biologic potential. Gene expression studies are made possible not only by the decoding of the human genome, but by the development of new technologies. The preeminent technology in this area, DNA microarrays, is helping to revolutionize the field of neuroscience. Rather than looking at one gene at a time, researchers using DNA microarrays can monitor the expression patterns of large numbers of genes simultaneously. Bridging the traditional gap between molecular neurobiology and systems neurobiology, DNA microarray technology has the potential to elevate molecular genetic studies of the nervous system to the system level. This book provides a comprehensive guide to the use of DNA microarrays in neuroscience and provides approaches that are applicable to other complex biological systems. Human nervous system tissue is remarkably complex. The number of cell types, the architecture, the developmental program, and the importance of environmental factors in development and functioning all pose particular challenges to the researcher using gene expression studies. After an overview of the technology, the book discusses array scanning and image application, statistical methods for array analysis, specific applications of gene expression studies in the central nervous system, the use of postmortem human tissue, and novel methods for using microarray data to develop hypotheses about regulatory networks.
Bradford Books imprint
The DNA microarrays are generally accepted as great new tools in biology and medicine, but they are still not sufficiently utilized in studying gene expression in complex tissues such as the mammalian central nervous system. This book is a long-awaited and much-anticipated source of basic information for neuroscientists who plan to use or already have begun to use the microarray technology. The value of this book is that it contains chapters prepared by experts that cover different aspects of this technology from a very basic introduction to microarray technology to the examples of its successful application in the developmental, integrative and clinical neurosciences. However, even to those who do not plan to use this approach, it could be of great help to understanding the language and appreciating the advances that will undoubtedly arrive.
Pasko Rakic, Professor of Neuroscience, Yale University School of Medicine
Gene expression profiling has clearly emerged as an effective new technology for studying the brain. This very useful volume provides the first comprehensive guide to this new technology for neuroscientists.
Fred H. Gage, Adler Professor, Laboratory of Genetics, The Salk Institute for Biological Studies
The nuts and bolts of utilizing gene microarray technology, from production to complex data analysis, are presented in an eminently digestible and technically current fashion. Having experienced the difficulties in fashioning a technical book, I am impressed with the thoroughness and attention to experimental and analytical detail that Geschwind and Gregg have incorporated with their collaborators.
Pat Levitt, Director, John F. Kennedy Center for Research on Human Development, Vanderbilt University.,