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DOI: http://dx.doi.org/10.7551/978-0-262-32621-6-ch156
Pages 963-964
First published 30 July 2014

In Vitro Reconstruction of Functional Membrane

Yutetsu Kuruma, Hideaki Matsubayashi, and Takuya Ueda

Abstract (Excerpt)

One feasible strategy in constructing an artificial cell is the assembly of biomolecules to fulfill the basic cellular reactions which necessary for cell alive (Luisi et al., 2006). In this strategy, membrane vesicle has been widely employed as a model cell compartment allowing internal biochemical reaction such as gene expression (Fujii et al., 2013), lipid biosynthesis (Kuruma et al., 2009), and so on. Although difficulties in constructing biochemically functional membrane composed of lipids and membrane proteins have limited the construction of a viable artificial cell, it has been reported that some kind of membrane protein can be spontaneously integrated into a lipid membrane during the translation reaction by ribosome (Ashley et al., 2012). This phenomenon can be efficiently applied for the construction of membrane protein architecture on the vesicle membrane. On the other hand, not all membrane proteins can spontaneously integrate in the native-like conformation. For example, if a membrane protein contains a large hydrophilic domain at the other side of membrane, this type protein requires a membrane translocation channel, which called as Sec Translocon (Luirink et al., 2005). Therefore, if the Sec translocon could be synthesized within the vesicle, any membrane protein should be synthesized as downstream reaction in the regulated membrane integration.